Western Blot:Article Title: TERRA R-loops connect and protect sister telomeres in mitosis.
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 mg/mL; Millipore), mouse anti-Flag F3165 (3.8 mg/mL, Sigma), rabbit anti- C19orf43 PA5-63805 (0.4 mg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 mg/mL; Proteintech), rabbit anti-TNKS1 762 (1 mg/mL),63 rabbit anti-RNaseH1 15606-AP (0.5 mg/mL, Proteintech), rabbit anti RNaseH2A PA5-78330 (0.82 mg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 mg/mL, Santa Cruz), or mouse anti-a-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 μg/mL; Millipore), mouse anti-Flag F3165 (3.8 μg/mL, Sigma), rabbit anti- C19orf43 PA5–63805 (0.4 μg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 μg/mL; Proteintech), rabbit anti-TNKS1 762 (1 μg/mL), rabbit anti-RNaseH1 15606-AP (0.5 μg/mL, Proteintech), rabbit anti RNaseH2A PA5–78330 (0.82 μg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 μg/mL, Santa Cruz), or mouse anti-α-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: Reduction of Nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration
Article Snippet: 563 The following primary antibodies used were: rat anti-Lamp2 (Abcam; ab13524; 1:100), mouse 564 anti-Cathepsin D (Abcam; ab6313; 1:200), goat anti-ChAT (Millipore; AB144P; 1:500), rabbit anti-Lamp1 565 (Abcam; ab24170; 1:400), chicken anti-GFAP (Abcam; ab4674; 1:2,000), mouse anti-NeuN (Millipore; 566 MAB377; 1:200), mouse anti-SMI32 (Covance; SMI-32P; 1:1,000), mouse anti-Flag (Sigma-Aldrich; 567 F3165; 1:2000), and chicken anti-GFP (Abcam; ab13970; 1:3,000).
Article Title: The in vivo functional significance of PUF hub partnerships in C. elegans germline stem cells
Article Snippet: For primary antibodies, blots were incubated overnight at 4°C at the following dilutions: mouse anti-FLAG (1:1000, M2 clone, Sigma-Aldrich, F3165), mouse anti-V5 (1:1000, Bio-Rad, MCA1360), mouse anti-actin (1:40,000, C4 clone, Millipore, MAB1501).
Article Title: A novel region within a conserved domain in ATG7 emerged in vertebrates
Article Snippet: The primary antibodies used were mouse anti-FLAG (Sigma, F3165, 1:5000), mouse anti-actin (Millipore, MAB1501, 1:15000), rabbit anti-LC3B (Cell Signaling, #2775, 1:1000), and rabbit anti-p62 (Cell Signaling, #5114, 1:1000).
Article Title: Endocytosis of the glutamate transporter 1 is regulated by Laforin and Malin: implications in Lafora Disease.
Article Snippet: Membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with the corresponding antibodies: guinea pig anti-GLT-1 (AB1783, Millipore); mouse anti-EAAT2 (sc-365634, Santa Cruz Biotechnologies); rabbit anti-Nedd4.2 (#4013, Cell Signaling); mouse anti-HA (H9658, Sigma); mouse anti-Flag (F3165, Sigma) and rabbit anti-GFP (210-PS-1GFP, Inmunokontackt).
Article Title: Tyrosine phosphatase activity is restricted by basic charge substituting mutation of substrates
Article Snippet: The following primary antibodies were used in this study: mouse anti-FLAG (F3165, MilliporeSigma), rabbit anti-FLAG (F7425, MilliporeSigma), rabbit anti-pY654-β-cat (ab59430, abcam), rabbit anti-Src (701396, Invitrogen), mouse anti-phosphotyrosine (clone PY20, P4110, MilliporeSigma), mouse anti-SHP1 (MA5-11669, Invitrogen), rabbit anti-β-cat (clone RM276, SAB5600086, MilliporeSigma), mouse anti-β-cat (610153, BD Bioscience), mouse anti-γ-catenin (610253, BD Bioscience), rabbit anti-AXIN2 (PA5-25331, Invitrogen), mouse anti-GAPDH (CB1001, MilliporeSigma).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc 05-724 (0.1 μg/ml; Millipore), mouse anti-Flag F3165 (3.8 μg/ml, Sigma), rabbit anti-C19orf43 PA5-63805 (0.4 μg/ml, Invitrogen), rabbit anti-TNKS1 762 (1 μg/ml) , rabbit anti-RNaseH1 15606-AP (0.5 μg/ml, Proteintech), or mouse anti-α-tubulin ascites (1:10000-1:20000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3000, Amersham).
Incubation:Article Title: TERRA R-loops connect and protect sister telomeres in mitosis.
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 mg/mL; Millipore), mouse anti-Flag F3165 (3.8 mg/mL, Sigma), rabbit anti- C19orf43 PA5-63805 (0.4 mg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 mg/mL; Proteintech), rabbit anti-TNKS1 762 (1 mg/mL),63 rabbit anti-RNaseH1 15606-AP (0.5 mg/mL, Proteintech), rabbit anti RNaseH2A PA5-78330 (0.82 mg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 mg/mL, Santa Cruz), or mouse anti-a-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 μg/mL; Millipore), mouse anti-Flag F3165 (3.8 μg/mL, Sigma), rabbit anti- C19orf43 PA5–63805 (0.4 μg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 μg/mL; Proteintech), rabbit anti-TNKS1 762 (1 μg/mL), rabbit anti-RNaseH1 15606-AP (0.5 μg/mL, Proteintech), rabbit anti RNaseH2A PA5–78330 (0.82 μg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 μg/mL, Santa Cruz), or mouse anti-α-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: Reduction of Nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration
Article Snippet: 563 The following primary antibodies used were: rat anti-Lamp2 (Abcam; ab13524; 1:100), mouse 564 anti-Cathepsin D (Abcam; ab6313; 1:200), goat anti-ChAT (Millipore; AB144P; 1:500), rabbit anti-Lamp1 565 (Abcam; ab24170; 1:400), chicken anti-GFAP (Abcam; ab4674; 1:2,000), mouse anti-NeuN (Millipore; 566 MAB377; 1:200), mouse anti-SMI32 (Covance; SMI-32P; 1:1,000), mouse anti-Flag (Sigma-Aldrich; 567 F3165; 1:2000), and chicken anti-GFP (Abcam; ab13970; 1:3,000).
Article Title: The in vivo functional significance of PUF hub partnerships in C. elegans germline stem cells
Article Snippet: For primary antibodies, blots were incubated overnight at 4°C at the following dilutions: mouse anti-FLAG (1:1000, M2 clone, Sigma-Aldrich, F3165), mouse anti-V5 (1:1000, Bio-Rad, MCA1360), mouse anti-actin (1:40,000, C4 clone, Millipore, MAB1501).
Article Title: A novel region within a conserved domain in ATG7 emerged in vertebrates
Article Snippet: The primary antibodies used were mouse anti-FLAG (Sigma, F3165, 1:5000), mouse anti-actin (Millipore, MAB1501, 1:15000), rabbit anti-LC3B (Cell Signaling, #2775, 1:1000), and rabbit anti-p62 (Cell Signaling, #5114, 1:1000).
Article Title: Endocytosis of the glutamate transporter 1 is regulated by Laforin and Malin: implications in Lafora Disease.
Article Snippet: Membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with the corresponding antibodies: guinea pig anti-GLT-1 (AB1783, Millipore); mouse anti-EAAT2 (sc-365634, Santa Cruz Biotechnologies); rabbit anti-Nedd4.2 (#4013, Cell Signaling); mouse anti-HA (H9658, Sigma); mouse anti-Flag (F3165, Sigma) and rabbit anti-GFP (210-PS-1GFP, Inmunokontackt).
Article Title: Tyrosine phosphatase activity is restricted by basic charge substituting mutation of substrates
Article Snippet: The following primary antibodies were used in this study: mouse anti-FLAG (F3165, MilliporeSigma), rabbit anti-FLAG (F7425, MilliporeSigma), rabbit anti-pY654-β-cat (ab59430, abcam), rabbit anti-Src (701396, Invitrogen), mouse anti-phosphotyrosine (clone PY20, P4110, MilliporeSigma), mouse anti-SHP1 (MA5-11669, Invitrogen), rabbit anti-β-cat (clone RM276, SAB5600086, MilliporeSigma), mouse anti-β-cat (610153, BD Bioscience), mouse anti-γ-catenin (610253, BD Bioscience), rabbit anti-AXIN2 (PA5-25331, Invitrogen), mouse anti-GAPDH (CB1001, MilliporeSigma).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc 05-724 (0.1 μg/ml; Millipore), mouse anti-Flag F3165 (3.8 μg/ml, Sigma), rabbit anti-C19orf43 PA5-63805 (0.4 μg/ml, Invitrogen), rabbit anti-TNKS1 762 (1 μg/ml) , rabbit anti-RNaseH1 15606-AP (0.5 μg/ml, Proteintech), or mouse anti-α-tubulin ascites (1:10000-1:20000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3000, Amersham).
Produced:Article Title: TERRA R-loops connect and protect sister telomeres in mitosis.
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 mg/mL; Millipore), mouse anti-Flag F3165 (3.8 mg/mL, Sigma), rabbit anti- C19orf43 PA5-63805 (0.4 mg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 mg/mL; Proteintech), rabbit anti-TNKS1 762 (1 mg/mL),63 rabbit anti-RNaseH1 15606-AP (0.5 mg/mL, Proteintech), rabbit anti RNaseH2A PA5-78330 (0.82 mg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 mg/mL, Santa Cruz), or mouse anti-a-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 μg/mL; Millipore), mouse anti-Flag F3165 (3.8 μg/mL, Sigma), rabbit anti- C19orf43 PA5–63805 (0.4 μg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 μg/mL; Proteintech), rabbit anti-TNKS1 762 (1 μg/mL), rabbit anti-RNaseH1 15606-AP (0.5 μg/mL, Proteintech), rabbit anti RNaseH2A PA5–78330 (0.82 μg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 μg/mL, Santa Cruz), or mouse anti-α-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: Reduction of Nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration
Article Snippet: 563 The following primary antibodies used were: rat anti-Lamp2 (Abcam; ab13524; 1:100), mouse 564 anti-Cathepsin D (Abcam; ab6313; 1:200), goat anti-ChAT (Millipore; AB144P; 1:500), rabbit anti-Lamp1 565 (Abcam; ab24170; 1:400), chicken anti-GFAP (Abcam; ab4674; 1:2,000), mouse anti-NeuN (Millipore; 566 MAB377; 1:200), mouse anti-SMI32 (Covance; SMI-32P; 1:1,000), mouse anti-Flag (Sigma-Aldrich; 567 F3165; 1:2000), and chicken anti-GFP (Abcam; ab13970; 1:3,000).
Article Title: The in vivo functional significance of PUF hub partnerships in C. elegans germline stem cells
Article Snippet: For primary antibodies, blots were incubated overnight at 4°C at the following dilutions: mouse anti-FLAG (1:1000, M2 clone, Sigma-Aldrich, F3165), mouse anti-V5 (1:1000, Bio-Rad, MCA1360), mouse anti-actin (1:40,000, C4 clone, Millipore, MAB1501).
Article Title: A novel region within a conserved domain in ATG7 emerged in vertebrates
Article Snippet: The primary antibodies used were mouse anti-FLAG (Sigma, F3165, 1:5000), mouse anti-actin (Millipore, MAB1501, 1:15000), rabbit anti-LC3B (Cell Signaling, #2775, 1:1000), and rabbit anti-p62 (Cell Signaling, #5114, 1:1000).
Article Title: Endocytosis of the glutamate transporter 1 is regulated by Laforin and Malin: implications in Lafora Disease.
Article Snippet: Membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with the corresponding antibodies: guinea pig anti-GLT-1 (AB1783, Millipore); mouse anti-EAAT2 (sc-365634, Santa Cruz Biotechnologies); rabbit anti-Nedd4.2 (#4013, Cell Signaling); mouse anti-HA (H9658, Sigma); mouse anti-Flag (F3165, Sigma) and rabbit anti-GFP (210-PS-1GFP, Inmunokontackt).
Article Title: Tyrosine phosphatase activity is restricted by basic charge substituting mutation of substrates
Article Snippet: The following primary antibodies were used in this study: mouse anti-FLAG (F3165, MilliporeSigma), rabbit anti-FLAG (F7425, MilliporeSigma), rabbit anti-pY654-β-cat (ab59430, abcam), rabbit anti-Src (701396, Invitrogen), mouse anti-phosphotyrosine (clone PY20, P4110, MilliporeSigma), mouse anti-SHP1 (MA5-11669, Invitrogen), rabbit anti-β-cat (clone RM276, SAB5600086, MilliporeSigma), mouse anti-β-cat (610153, BD Bioscience), mouse anti-γ-catenin (610253, BD Bioscience), rabbit anti-AXIN2 (PA5-25331, Invitrogen), mouse anti-GAPDH (CB1001, MilliporeSigma).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc 05-724 (0.1 μg/ml; Millipore), mouse anti-Flag F3165 (3.8 μg/ml, Sigma), rabbit anti-C19orf43 PA5-63805 (0.4 μg/ml, Invitrogen), rabbit anti-TNKS1 762 (1 μg/ml) , rabbit anti-RNaseH1 15606-AP (0.5 μg/ml, Proteintech), or mouse anti-α-tubulin ascites (1:10000-1:20000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3000, Amersham).
Recombinant:Article Title: TERRA R-loops connect and protect sister telomeres in mitosis.
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 mg/mL; Millipore), mouse anti-Flag F3165 (3.8 mg/mL, Sigma), rabbit anti- C19orf43 PA5-63805 (0.4 mg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 mg/mL; Proteintech), rabbit anti-TNKS1 762 (1 mg/mL),63 rabbit anti-RNaseH1 15606-AP (0.5 mg/mL, Proteintech), rabbit anti RNaseH2A PA5-78330 (0.82 mg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 mg/mL, Santa Cruz), or mouse anti-a-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 μg/mL; Millipore), mouse anti-Flag F3165 (3.8 μg/mL, Sigma), rabbit anti- C19orf43 PA5–63805 (0.4 μg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 μg/mL; Proteintech), rabbit anti-TNKS1 762 (1 μg/mL), rabbit anti-RNaseH1 15606-AP (0.5 μg/mL, Proteintech), rabbit anti RNaseH2A PA5–78330 (0.82 μg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 μg/mL, Santa Cruz), or mouse anti-α-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: Reduction of Nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration
Article Snippet: 563 The following primary antibodies used were: rat anti-Lamp2 (Abcam; ab13524; 1:100), mouse 564 anti-Cathepsin D (Abcam; ab6313; 1:200), goat anti-ChAT (Millipore; AB144P; 1:500), rabbit anti-Lamp1 565 (Abcam; ab24170; 1:400), chicken anti-GFAP (Abcam; ab4674; 1:2,000), mouse anti-NeuN (Millipore; 566 MAB377; 1:200), mouse anti-SMI32 (Covance; SMI-32P; 1:1,000), mouse anti-Flag (Sigma-Aldrich; 567 F3165; 1:2000), and chicken anti-GFP (Abcam; ab13970; 1:3,000).
Article Title: The in vivo functional significance of PUF hub partnerships in C. elegans germline stem cells
Article Snippet: For primary antibodies, blots were incubated overnight at 4°C at the following dilutions: mouse anti-FLAG (1:1000, M2 clone, Sigma-Aldrich, F3165), mouse anti-V5 (1:1000, Bio-Rad, MCA1360), mouse anti-actin (1:40,000, C4 clone, Millipore, MAB1501).
Article Title: A novel region within a conserved domain in ATG7 emerged in vertebrates
Article Snippet: The primary antibodies used were mouse anti-FLAG (Sigma, F3165, 1:5000), mouse anti-actin (Millipore, MAB1501, 1:15000), rabbit anti-LC3B (Cell Signaling, #2775, 1:1000), and rabbit anti-p62 (Cell Signaling, #5114, 1:1000).
Article Title: Endocytosis of the glutamate transporter 1 is regulated by Laforin and Malin: implications in Lafora Disease.
Article Snippet: Membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with the corresponding antibodies: guinea pig anti-GLT-1 (AB1783, Millipore); mouse anti-EAAT2 (sc-365634, Santa Cruz Biotechnologies); rabbit anti-Nedd4.2 (#4013, Cell Signaling); mouse anti-HA (H9658, Sigma); mouse anti-Flag (F3165, Sigma) and rabbit anti-GFP (210-PS-1GFP, Inmunokontackt).
Article Title: Tyrosine phosphatase activity is restricted by basic charge substituting mutation of substrates
Article Snippet: The following primary antibodies were used in this study: mouse anti-FLAG (F3165, MilliporeSigma), rabbit anti-FLAG (F7425, MilliporeSigma), rabbit anti-pY654-β-cat (ab59430, abcam), rabbit anti-Src (701396, Invitrogen), mouse anti-phosphotyrosine (clone PY20, P4110, MilliporeSigma), mouse anti-SHP1 (MA5-11669, Invitrogen), rabbit anti-β-cat (clone RM276, SAB5600086, MilliporeSigma), mouse anti-β-cat (610153, BD Bioscience), mouse anti-γ-catenin (610253, BD Bioscience), rabbit anti-AXIN2 (PA5-25331, Invitrogen), mouse anti-GAPDH (CB1001, MilliporeSigma).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc 05-724 (0.1 μg/ml; Millipore), mouse anti-Flag F3165 (3.8 μg/ml, Sigma), rabbit anti-C19orf43 PA5-63805 (0.4 μg/ml, Invitrogen), rabbit anti-TNKS1 762 (1 μg/ml) , rabbit anti-RNaseH1 15606-AP (0.5 μg/ml, Proteintech), or mouse anti-α-tubulin ascites (1:10000-1:20000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3000, Amersham).
Cell Culture:Article Title: TERRA R-loops connect and protect sister telomeres in mitosis.
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 mg/mL; Millipore), mouse anti-Flag F3165 (3.8 mg/mL, Sigma), rabbit anti- C19orf43 PA5-63805 (0.4 mg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 mg/mL; Proteintech), rabbit anti-TNKS1 762 (1 mg/mL),63 rabbit anti-RNaseH1 15606-AP (0.5 mg/mL, Proteintech), rabbit anti RNaseH2A PA5-78330 (0.82 mg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 mg/mL, Santa Cruz), or mouse anti-a-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 μg/mL; Millipore), mouse anti-Flag F3165 (3.8 μg/mL, Sigma), rabbit anti- C19orf43 PA5–63805 (0.4 μg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 μg/mL; Proteintech), rabbit anti-TNKS1 762 (1 μg/mL), rabbit anti-RNaseH1 15606-AP (0.5 μg/mL, Proteintech), rabbit anti RNaseH2A PA5–78330 (0.82 μg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 μg/mL, Santa Cruz), or mouse anti-α-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: Reduction of Nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration
Article Snippet: 563 The following primary antibodies used were: rat anti-Lamp2 (Abcam; ab13524; 1:100), mouse 564 anti-Cathepsin D (Abcam; ab6313; 1:200), goat anti-ChAT (Millipore; AB144P; 1:500), rabbit anti-Lamp1 565 (Abcam; ab24170; 1:400), chicken anti-GFAP (Abcam; ab4674; 1:2,000), mouse anti-NeuN (Millipore; 566 MAB377; 1:200), mouse anti-SMI32 (Covance; SMI-32P; 1:1,000), mouse anti-Flag (Sigma-Aldrich; 567 F3165; 1:2000), and chicken anti-GFP (Abcam; ab13970; 1:3,000).
Article Title: The in vivo functional significance of PUF hub partnerships in C. elegans germline stem cells
Article Snippet: For primary antibodies, blots were incubated overnight at 4°C at the following dilutions: mouse anti-FLAG (1:1000, M2 clone, Sigma-Aldrich, F3165), mouse anti-V5 (1:1000, Bio-Rad, MCA1360), mouse anti-actin (1:40,000, C4 clone, Millipore, MAB1501).
Article Title: A novel region within a conserved domain in ATG7 emerged in vertebrates
Article Snippet: The primary antibodies used were mouse anti-FLAG (Sigma, F3165, 1:5000), mouse anti-actin (Millipore, MAB1501, 1:15000), rabbit anti-LC3B (Cell Signaling, #2775, 1:1000), and rabbit anti-p62 (Cell Signaling, #5114, 1:1000).
Article Title: Endocytosis of the glutamate transporter 1 is regulated by Laforin and Malin: implications in Lafora Disease.
Article Snippet: Membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with the corresponding antibodies: guinea pig anti-GLT-1 (AB1783, Millipore); mouse anti-EAAT2 (sc-365634, Santa Cruz Biotechnologies); rabbit anti-Nedd4.2 (#4013, Cell Signaling); mouse anti-HA (H9658, Sigma); mouse anti-Flag (F3165, Sigma) and rabbit anti-GFP (210-PS-1GFP, Inmunokontackt).
Article Title: Tyrosine phosphatase activity is restricted by basic charge substituting mutation of substrates
Article Snippet: The following primary antibodies were used in this study: mouse anti-FLAG (F3165, MilliporeSigma), rabbit anti-FLAG (F7425, MilliporeSigma), rabbit anti-pY654-β-cat (ab59430, abcam), rabbit anti-Src (701396, Invitrogen), mouse anti-phosphotyrosine (clone PY20, P4110, MilliporeSigma), mouse anti-SHP1 (MA5-11669, Invitrogen), rabbit anti-β-cat (clone RM276, SAB5600086, MilliporeSigma), mouse anti-β-cat (610153, BD Bioscience), mouse anti-γ-catenin (610253, BD Bioscience), rabbit anti-AXIN2 (PA5-25331, Invitrogen), mouse anti-GAPDH (CB1001, MilliporeSigma).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc 05-724 (0.1 μg/ml; Millipore), mouse anti-Flag F3165 (3.8 μg/ml, Sigma), rabbit anti-C19orf43 PA5-63805 (0.4 μg/ml, Invitrogen), rabbit anti-TNKS1 762 (1 μg/ml) , rabbit anti-RNaseH1 15606-AP (0.5 μg/ml, Proteintech), or mouse anti-α-tubulin ascites (1:10000-1:20000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3000, Amersham).
CRISPR:Article Title: TERRA R-loops connect and protect sister telomeres in mitosis.
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 mg/mL; Millipore), mouse anti-Flag F3165 (3.8 mg/mL, Sigma), rabbit anti- C19orf43 PA5-63805 (0.4 mg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 mg/mL; Proteintech), rabbit anti-TNKS1 762 (1 mg/mL),63 rabbit anti-RNaseH1 15606-AP (0.5 mg/mL, Proteintech), rabbit anti RNaseH2A PA5-78330 (0.82 mg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 mg/mL, Santa Cruz), or mouse anti-a-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 μg/mL; Millipore), mouse anti-Flag F3165 (3.8 μg/mL, Sigma), rabbit anti- C19orf43 PA5–63805 (0.4 μg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 μg/mL; Proteintech), rabbit anti-TNKS1 762 (1 μg/mL), rabbit anti-RNaseH1 15606-AP (0.5 μg/mL, Proteintech), rabbit anti RNaseH2A PA5–78330 (0.82 μg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 μg/mL, Santa Cruz), or mouse anti-α-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: Reduction of Nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration
Article Snippet: 563 The following primary antibodies used were: rat anti-Lamp2 (Abcam; ab13524; 1:100), mouse 564 anti-Cathepsin D (Abcam; ab6313; 1:200), goat anti-ChAT (Millipore; AB144P; 1:500), rabbit anti-Lamp1 565 (Abcam; ab24170; 1:400), chicken anti-GFAP (Abcam; ab4674; 1:2,000), mouse anti-NeuN (Millipore; 566 MAB377; 1:200), mouse anti-SMI32 (Covance; SMI-32P; 1:1,000), mouse anti-Flag (Sigma-Aldrich; 567 F3165; 1:2000), and chicken anti-GFP (Abcam; ab13970; 1:3,000).
Article Title: The in vivo functional significance of PUF hub partnerships in C. elegans germline stem cells
Article Snippet: For primary antibodies, blots were incubated overnight at 4°C at the following dilutions: mouse anti-FLAG (1:1000, M2 clone, Sigma-Aldrich, F3165), mouse anti-V5 (1:1000, Bio-Rad, MCA1360), mouse anti-actin (1:40,000, C4 clone, Millipore, MAB1501).
Article Title: A novel region within a conserved domain in ATG7 emerged in vertebrates
Article Snippet: The primary antibodies used were mouse anti-FLAG (Sigma, F3165, 1:5000), mouse anti-actin (Millipore, MAB1501, 1:15000), rabbit anti-LC3B (Cell Signaling, #2775, 1:1000), and rabbit anti-p62 (Cell Signaling, #5114, 1:1000).
Article Title: Endocytosis of the glutamate transporter 1 is regulated by Laforin and Malin: implications in Lafora Disease.
Article Snippet: Membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with the corresponding antibodies: guinea pig anti-GLT-1 (AB1783, Millipore); mouse anti-EAAT2 (sc-365634, Santa Cruz Biotechnologies); rabbit anti-Nedd4.2 (#4013, Cell Signaling); mouse anti-HA (H9658, Sigma); mouse anti-Flag (F3165, Sigma) and rabbit anti-GFP (210-PS-1GFP, Inmunokontackt).
Article Title: Tyrosine phosphatase activity is restricted by basic charge substituting mutation of substrates
Article Snippet: The following primary antibodies were used in this study: mouse anti-FLAG (F3165, MilliporeSigma), rabbit anti-FLAG (F7425, MilliporeSigma), rabbit anti-pY654-β-cat (ab59430, abcam), rabbit anti-Src (701396, Invitrogen), mouse anti-phosphotyrosine (clone PY20, P4110, MilliporeSigma), mouse anti-SHP1 (MA5-11669, Invitrogen), rabbit anti-β-cat (clone RM276, SAB5600086, MilliporeSigma), mouse anti-β-cat (610153, BD Bioscience), mouse anti-γ-catenin (610253, BD Bioscience), rabbit anti-AXIN2 (PA5-25331, Invitrogen), mouse anti-GAPDH (CB1001, MilliporeSigma).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc 05-724 (0.1 μg/ml; Millipore), mouse anti-Flag F3165 (3.8 μg/ml, Sigma), rabbit anti-C19orf43 PA5-63805 (0.4 μg/ml, Invitrogen), rabbit anti-TNKS1 762 (1 μg/ml) , rabbit anti-RNaseH1 15606-AP (0.5 μg/ml, Proteintech), or mouse anti-α-tubulin ascites (1:10000-1:20000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3000, Amersham).
Plasmid Preparation:Article Title: TERRA R-loops connect and protect sister telomeres in mitosis.
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 mg/mL; Millipore), mouse anti-Flag F3165 (3.8 mg/mL, Sigma), rabbit anti- C19orf43 PA5-63805 (0.4 mg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 mg/mL; Proteintech), rabbit anti-TNKS1 762 (1 mg/mL),63 rabbit anti-RNaseH1 15606-AP (0.5 mg/mL, Proteintech), rabbit anti RNaseH2A PA5-78330 (0.82 mg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 mg/mL, Santa Cruz), or mouse anti-a-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 μg/mL; Millipore), mouse anti-Flag F3165 (3.8 μg/mL, Sigma), rabbit anti- C19orf43 PA5–63805 (0.4 μg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 μg/mL; Proteintech), rabbit anti-TNKS1 762 (1 μg/mL), rabbit anti-RNaseH1 15606-AP (0.5 μg/mL, Proteintech), rabbit anti RNaseH2A PA5–78330 (0.82 μg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 μg/mL, Santa Cruz), or mouse anti-α-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: Reduction of Nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration
Article Snippet: 563 The following primary antibodies used were: rat anti-Lamp2 (Abcam; ab13524; 1:100), mouse 564 anti-Cathepsin D (Abcam; ab6313; 1:200), goat anti-ChAT (Millipore; AB144P; 1:500), rabbit anti-Lamp1 565 (Abcam; ab24170; 1:400), chicken anti-GFAP (Abcam; ab4674; 1:2,000), mouse anti-NeuN (Millipore; 566 MAB377; 1:200), mouse anti-SMI32 (Covance; SMI-32P; 1:1,000), mouse anti-Flag (Sigma-Aldrich; 567 F3165; 1:2000), and chicken anti-GFP (Abcam; ab13970; 1:3,000).
Article Title: The in vivo functional significance of PUF hub partnerships in C. elegans germline stem cells
Article Snippet: For primary antibodies, blots were incubated overnight at 4°C at the following dilutions: mouse anti-FLAG (1:1000, M2 clone, Sigma-Aldrich, F3165), mouse anti-V5 (1:1000, Bio-Rad, MCA1360), mouse anti-actin (1:40,000, C4 clone, Millipore, MAB1501).
Article Title: A novel region within a conserved domain in ATG7 emerged in vertebrates
Article Snippet: The primary antibodies used were mouse anti-FLAG (Sigma, F3165, 1:5000), mouse anti-actin (Millipore, MAB1501, 1:15000), rabbit anti-LC3B (Cell Signaling, #2775, 1:1000), and rabbit anti-p62 (Cell Signaling, #5114, 1:1000).
Article Title: Endocytosis of the glutamate transporter 1 is regulated by Laforin and Malin: implications in Lafora Disease.
Article Snippet: Membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with the corresponding antibodies: guinea pig anti-GLT-1 (AB1783, Millipore); mouse anti-EAAT2 (sc-365634, Santa Cruz Biotechnologies); rabbit anti-Nedd4.2 (#4013, Cell Signaling); mouse anti-HA (H9658, Sigma); mouse anti-Flag (F3165, Sigma) and rabbit anti-GFP (210-PS-1GFP, Inmunokontackt).
Article Title: Tyrosine phosphatase activity is restricted by basic charge substituting mutation of substrates
Article Snippet: The following primary antibodies were used in this study: mouse anti-FLAG (F3165, MilliporeSigma), rabbit anti-FLAG (F7425, MilliporeSigma), rabbit anti-pY654-β-cat (ab59430, abcam), rabbit anti-Src (701396, Invitrogen), mouse anti-phosphotyrosine (clone PY20, P4110, MilliporeSigma), mouse anti-SHP1 (MA5-11669, Invitrogen), rabbit anti-β-cat (clone RM276, SAB5600086, MilliporeSigma), mouse anti-β-cat (610153, BD Bioscience), mouse anti-γ-catenin (610253, BD Bioscience), rabbit anti-AXIN2 (PA5-25331, Invitrogen), mouse anti-GAPDH (CB1001, MilliporeSigma).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc 05-724 (0.1 μg/ml; Millipore), mouse anti-Flag F3165 (3.8 μg/ml, Sigma), rabbit anti-C19orf43 PA5-63805 (0.4 μg/ml, Invitrogen), rabbit anti-TNKS1 762 (1 μg/ml) , rabbit anti-RNaseH1 15606-AP (0.5 μg/ml, Proteintech), or mouse anti-α-tubulin ascites (1:10000-1:20000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3000, Amersham).
Software:Article Title: TERRA R-loops connect and protect sister telomeres in mitosis.
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 mg/mL; Millipore), mouse anti-Flag F3165 (3.8 mg/mL, Sigma), rabbit anti- C19orf43 PA5-63805 (0.4 mg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 mg/mL; Proteintech), rabbit anti-TNKS1 762 (1 mg/mL),63 rabbit anti-RNaseH1 15606-AP (0.5 mg/mL, Proteintech), rabbit anti RNaseH2A PA5-78330 (0.82 mg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 mg/mL, Santa Cruz), or mouse anti-a-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 μg/mL; Millipore), mouse anti-Flag F3165 (3.8 μg/mL, Sigma), rabbit anti- C19orf43 PA5–63805 (0.4 μg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 μg/mL; Proteintech), rabbit anti-TNKS1 762 (1 μg/mL), rabbit anti-RNaseH1 15606-AP (0.5 μg/mL, Proteintech), rabbit anti RNaseH2A PA5–78330 (0.82 μg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 μg/mL, Santa Cruz), or mouse anti-α-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: Reduction of Nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration
Article Snippet: 563 The following primary antibodies used were: rat anti-Lamp2 (Abcam; ab13524; 1:100), mouse 564 anti-Cathepsin D (Abcam; ab6313; 1:200), goat anti-ChAT (Millipore; AB144P; 1:500), rabbit anti-Lamp1 565 (Abcam; ab24170; 1:400), chicken anti-GFAP (Abcam; ab4674; 1:2,000), mouse anti-NeuN (Millipore; 566 MAB377; 1:200), mouse anti-SMI32 (Covance; SMI-32P; 1:1,000), mouse anti-Flag (Sigma-Aldrich; 567 F3165; 1:2000), and chicken anti-GFP (Abcam; ab13970; 1:3,000).
Article Title: The in vivo functional significance of PUF hub partnerships in C. elegans germline stem cells
Article Snippet: For primary antibodies, blots were incubated overnight at 4°C at the following dilutions: mouse anti-FLAG (1:1000, M2 clone, Sigma-Aldrich, F3165), mouse anti-V5 (1:1000, Bio-Rad, MCA1360), mouse anti-actin (1:40,000, C4 clone, Millipore, MAB1501).
Article Title: A novel region within a conserved domain in ATG7 emerged in vertebrates
Article Snippet: The primary antibodies used were mouse anti-FLAG (Sigma, F3165, 1:5000), mouse anti-actin (Millipore, MAB1501, 1:15000), rabbit anti-LC3B (Cell Signaling, #2775, 1:1000), and rabbit anti-p62 (Cell Signaling, #5114, 1:1000).
Article Title: Endocytosis of the glutamate transporter 1 is regulated by Laforin and Malin: implications in Lafora Disease.
Article Snippet: Membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with the corresponding antibodies: guinea pig anti-GLT-1 (AB1783, Millipore); mouse anti-EAAT2 (sc-365634, Santa Cruz Biotechnologies); rabbit anti-Nedd4.2 (#4013, Cell Signaling); mouse anti-HA (H9658, Sigma); mouse anti-Flag (F3165, Sigma) and rabbit anti-GFP (210-PS-1GFP, Inmunokontackt).
Article Title: Tyrosine phosphatase activity is restricted by basic charge substituting mutation of substrates
Article Snippet: The following primary antibodies were used in this study: mouse anti-FLAG (F3165, MilliporeSigma), rabbit anti-FLAG (F7425, MilliporeSigma), rabbit anti-pY654-β-cat (ab59430, abcam), rabbit anti-Src (701396, Invitrogen), mouse anti-phosphotyrosine (clone PY20, P4110, MilliporeSigma), mouse anti-SHP1 (MA5-11669, Invitrogen), rabbit anti-β-cat (clone RM276, SAB5600086, MilliporeSigma), mouse anti-β-cat (610153, BD Bioscience), mouse anti-γ-catenin (610253, BD Bioscience), rabbit anti-AXIN2 (PA5-25331, Invitrogen), mouse anti-GAPDH (CB1001, MilliporeSigma).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc 05-724 (0.1 μg/ml; Millipore), mouse anti-Flag F3165 (3.8 μg/ml, Sigma), rabbit anti-C19orf43 PA5-63805 (0.4 μg/ml, Invitrogen), rabbit anti-TNKS1 762 (1 μg/ml) , rabbit anti-RNaseH1 15606-AP (0.5 μg/ml, Proteintech), or mouse anti-α-tubulin ascites (1:10000-1:20000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3000, Amersham).
Blocking Assay:Article Title: TERRA R-loops connect and protect sister telomeres in mitosis.
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 mg/mL; Millipore), mouse anti-Flag F3165 (3.8 mg/mL, Sigma), rabbit anti- C19orf43 PA5-63805 (0.4 mg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 mg/mL; Proteintech), rabbit anti-TNKS1 762 (1 mg/mL),63 rabbit anti-RNaseH1 15606-AP (0.5 mg/mL, Proteintech), rabbit anti RNaseH2A PA5-78330 (0.82 mg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 mg/mL, Santa Cruz), or mouse anti-a-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 μg/mL; Millipore), mouse anti-Flag F3165 (3.8 μg/mL, Sigma), rabbit anti- C19orf43 PA5–63805 (0.4 μg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 μg/mL; Proteintech), rabbit anti-TNKS1 762 (1 μg/mL), rabbit anti-RNaseH1 15606-AP (0.5 μg/mL, Proteintech), rabbit anti RNaseH2A PA5–78330 (0.82 μg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 μg/mL, Santa Cruz), or mouse anti-α-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: Reduction of Nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration
Article Snippet: 563 The following primary antibodies used were: rat anti-Lamp2 (Abcam; ab13524; 1:100), mouse 564 anti-Cathepsin D (Abcam; ab6313; 1:200), goat anti-ChAT (Millipore; AB144P; 1:500), rabbit anti-Lamp1 565 (Abcam; ab24170; 1:400), chicken anti-GFAP (Abcam; ab4674; 1:2,000), mouse anti-NeuN (Millipore; 566 MAB377; 1:200), mouse anti-SMI32 (Covance; SMI-32P; 1:1,000), mouse anti-Flag (Sigma-Aldrich; 567 F3165; 1:2000), and chicken anti-GFP (Abcam; ab13970; 1:3,000).
Article Title: The in vivo functional significance of PUF hub partnerships in C. elegans germline stem cells
Article Snippet: For primary antibodies, blots were incubated overnight at 4°C at the following dilutions: mouse anti-FLAG (1:1000, M2 clone, Sigma-Aldrich, F3165), mouse anti-V5 (1:1000, Bio-Rad, MCA1360), mouse anti-actin (1:40,000, C4 clone, Millipore, MAB1501).
Article Title: A novel region within a conserved domain in ATG7 emerged in vertebrates
Article Snippet: The primary antibodies used were mouse anti-FLAG (Sigma, F3165, 1:5000), mouse anti-actin (Millipore, MAB1501, 1:15000), rabbit anti-LC3B (Cell Signaling, #2775, 1:1000), and rabbit anti-p62 (Cell Signaling, #5114, 1:1000).
Article Title: Endocytosis of the glutamate transporter 1 is regulated by Laforin and Malin: implications in Lafora Disease.
Article Snippet: Membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with the corresponding antibodies: guinea pig anti-GLT-1 (AB1783, Millipore); mouse anti-EAAT2 (sc-365634, Santa Cruz Biotechnologies); rabbit anti-Nedd4.2 (#4013, Cell Signaling); mouse anti-HA (H9658, Sigma); mouse anti-Flag (F3165, Sigma) and rabbit anti-GFP (210-PS-1GFP, Inmunokontackt).
Article Title: Tyrosine phosphatase activity is restricted by basic charge substituting mutation of substrates
Article Snippet: The following primary antibodies were used in this study: mouse anti-FLAG (F3165, MilliporeSigma), rabbit anti-FLAG (F7425, MilliporeSigma), rabbit anti-pY654-β-cat (ab59430, abcam), rabbit anti-Src (701396, Invitrogen), mouse anti-phosphotyrosine (clone PY20, P4110, MilliporeSigma), mouse anti-SHP1 (MA5-11669, Invitrogen), rabbit anti-β-cat (clone RM276, SAB5600086, MilliporeSigma), mouse anti-β-cat (610153, BD Bioscience), mouse anti-γ-catenin (610253, BD Bioscience), rabbit anti-AXIN2 (PA5-25331, Invitrogen), mouse anti-GAPDH (CB1001, MilliporeSigma).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc 05-724 (0.1 μg/ml; Millipore), mouse anti-Flag F3165 (3.8 μg/ml, Sigma), rabbit anti-C19orf43 PA5-63805 (0.4 μg/ml, Invitrogen), rabbit anti-TNKS1 762 (1 μg/ml) , rabbit anti-RNaseH1 15606-AP (0.5 μg/ml, Proteintech), or mouse anti-α-tubulin ascites (1:10000-1:20000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3000, Amersham).
Saline:Article Title: TERRA R-loops connect and protect sister telomeres in mitosis.
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 mg/mL; Millipore), mouse anti-Flag F3165 (3.8 mg/mL, Sigma), rabbit anti- C19orf43 PA5-63805 (0.4 mg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 mg/mL; Proteintech), rabbit anti-TNKS1 762 (1 mg/mL),63 rabbit anti-RNaseH1 15606-AP (0.5 mg/mL, Proteintech), rabbit anti RNaseH2A PA5-78330 (0.82 mg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 mg/mL, Santa Cruz), or mouse anti-a-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc05-724 (0.1 μg/mL; Millipore), mouse anti-Flag F3165 (3.8 μg/mL, Sigma), rabbit anti- C19orf43 PA5–63805 (0.4 μg/mL, Invitrogen), rabbit anti C19orf43 19420-1-AP (1.1 μg/mL; Proteintech), rabbit anti-TNKS1 762 (1 μg/mL), rabbit anti-RNaseH1 15606-AP (0.5 μg/mL, Proteintech), rabbit anti RNaseH2A PA5–78330 (0.82 μg/mL, Invitrogen), rabbit anti-RAD51 (H-92) sc-8349 (0.4 μg/mL, Santa Cruz), or mouse anti-α-tubulin ascites (1:20,000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3,000, Amersham).
Article Title: Reduction of Nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration
Article Snippet: 563 The following primary antibodies used were: rat anti-Lamp2 (Abcam; ab13524; 1:100), mouse 564 anti-Cathepsin D (Abcam; ab6313; 1:200), goat anti-ChAT (Millipore; AB144P; 1:500), rabbit anti-Lamp1 565 (Abcam; ab24170; 1:400), chicken anti-GFAP (Abcam; ab4674; 1:2,000), mouse anti-NeuN (Millipore; 566 MAB377; 1:200), mouse anti-SMI32 (Covance; SMI-32P; 1:1,000), mouse anti-Flag (Sigma-Aldrich; 567 F3165; 1:2000), and chicken anti-GFP (Abcam; ab13970; 1:3,000).
Article Title: The in vivo functional significance of PUF hub partnerships in C. elegans germline stem cells
Article Snippet: For primary antibodies, blots were incubated overnight at 4°C at the following dilutions: mouse anti-FLAG (1:1000, M2 clone, Sigma-Aldrich, F3165), mouse anti-V5 (1:1000, Bio-Rad, MCA1360), mouse anti-actin (1:40,000, C4 clone, Millipore, MAB1501).
Article Title: A novel region within a conserved domain in ATG7 emerged in vertebrates
Article Snippet: The primary antibodies used were mouse anti-FLAG (Sigma, F3165, 1:5000), mouse anti-actin (Millipore, MAB1501, 1:15000), rabbit anti-LC3B (Cell Signaling, #2775, 1:1000), and rabbit anti-p62 (Cell Signaling, #5114, 1:1000).
Article Title: Endocytosis of the glutamate transporter 1 is regulated by Laforin and Malin: implications in Lafora Disease.
Article Snippet: Membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with the corresponding antibodies: guinea pig anti-GLT-1 (AB1783, Millipore); mouse anti-EAAT2 (sc-365634, Santa Cruz Biotechnologies); rabbit anti-Nedd4.2 (#4013, Cell Signaling); mouse anti-HA (H9658, Sigma); mouse anti-Flag (F3165, Sigma) and rabbit anti-GFP (210-PS-1GFP, Inmunokontackt).
Article Title: Tyrosine phosphatase activity is restricted by basic charge substituting mutation of substrates
Article Snippet: The following primary antibodies were used in this study: mouse anti-FLAG (F3165, MilliporeSigma), rabbit anti-FLAG (F7425, MilliporeSigma), rabbit anti-pY654-β-cat (ab59430, abcam), rabbit anti-Src (701396, Invitrogen), mouse anti-phosphotyrosine (clone PY20, P4110, MilliporeSigma), mouse anti-SHP1 (MA5-11669, Invitrogen), rabbit anti-β-cat (clone RM276, SAB5600086, MilliporeSigma), mouse anti-β-cat (610153, BD Bioscience), mouse anti-γ-catenin (610253, BD Bioscience), rabbit anti-AXIN2 (PA5-25331, Invitrogen), mouse anti-GAPDH (CB1001, MilliporeSigma).
Article Title: TERRA R-loops connect and protect sister telomeres in mitosis
Article Snippet: Immunoblots were incubated separately with the following primary antibodies: mouse anti-Myc 05-724 (0.1 μg/ml; Millipore), mouse anti-Flag F3165 (3.8 μg/ml, Sigma), rabbit anti-C19orf43 PA5-63805 (0.4 μg/ml, Invitrogen), rabbit anti-TNKS1 762 (1 μg/ml) , rabbit anti-RNaseH1 15606-AP (0.5 μg/ml, Proteintech), or mouse anti-α-tubulin ascites (1:10000-1:20000, Sigma), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (1:3000, Amersham).
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Figure 1 A , and mouse mP1 protamine gene fragment containing polyadenylation signal. The PvuI/XbaI fragment excised from the vector was used for random DNA insertion in C57BL/6J mouse pronuclei to produce F 0 founders. B , immunoblotting of PDE5r in mouse retinas, line #22. Retinal extracts from PDE5r Tg and WT sibling were analyzed by Western immunoblotting probed by anti-PDE5A antibody as described under “Experimental procedures”. C , immunofluorescence of PDE5r probed using anti-FLAG antibody ( green ) in cryosections of PDE5r Tg ( upper panel ) and a WT sibling ( bottom panel); in the right part of each panel, nuclei in the same image frame are counterstained with TO-PRO-3 ( pseudo-blue ). D , Left panel. PDE5r immunofluorescence ( pseudo-cyan ) in rod outer segments; counterstained by anti-CNG1α antibody ( red ) to mark ROS plasma membrane and TOPRO3 ( pseudo-yellow ) to mark nuclei. Right panel. PDE5r Tg ROS are shown under higher magnification; PDE5r ( pseudo-cyan ) counterstained by anti-CNG1α antibody ( red ). E , PDE5r localization in ROS in comparison with PDE6. PDE5r Tg retina section was probed with anti-FLAG mouse antibody to mark PDE5r localization ( left panel, green fluorescence ) and anti-PDE6α rabbit antibody ( middle panel, pseudo - magenta ); the merged images are shown in the rightmost panel. F and G , retinal morphology in WT and PDE5r Tg mouse retinas at 6 months of age. F , representative optical coherence tomography (OCT) images of WT and PDE5r Tg mouse retinas. G , representative hematoxylin/eosin-stained aldehyde-fixed retinal cross-sections of WT and PDE5r Tg mouse retinas. Here and further the retinal histological layers are marked as follows: cDNA, complementary DNA; CHR, choroid; CNG, cyclic-nucleotide gated channel; ELM, external limiting membrane; INL, inner nuclear layer; IPL, inner plexiform layer; IS, rod inner segment layer; ONL, outer nuclear layer; OPL, outer plexiform layer; PDE, phosphodiesterase; PDE5r, recombinant PDE5; RGC, retinal ganglion cell layer; ROS, rod outer segment layer, RPE, retinal pigment epithelium. " width="100%" height="100%">
